Peanut Science December 01, 2016
Roots and pods of field-grown peanut were sampled at three developmental stages and a total of seven bacterial strains and one toxigenic Aspergillus flavus isolates were isolated from the geocarposphere. The biocontrol potential of each bacterial strain was tested against growth and Afl-B1 production potential of toxigenic A. flavus. In greenhouse experiments, co-inoculation with toxigenic A. flavus and geocarposphere bacteria in root regions of 1- to 2-week-old peanut plants resulted in the lower synthesis of Afl-B1 in the peanut kernels at maturity. Of the seven bacterial strains tested, four strains showed a reduction in aflatoxins production in varying extents. Pre-inoculation of bacterial strains (1 day earlier) resulted in greater inhibition of aflatoxin accumulation than those bacterial strains introduced 1 day after inoculation of toxigenic A. flavus strain. Bacillus megaterium showed maximum inhibition of aflatoxin biosynthesis, as compared to remaining three potential bacterial strains.